Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1

Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2

Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3

Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4

Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
PUBLICATIONS TRENDING NOW
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.

February 2023: CyTOF for CAR Therapy
March 2022: Maxpar Direct Immune Profiling Assay
January 2022: COVID-19 research update
November 2021: CAR T cells
July 2021: The value of high-parameter cytometry
April 2021: Immuno-oncology
January 2021: Cancer vaccine development
November 2020: Vaccines for infectious disease
July 2020: COVID-19 disease research
Browse the latest research highlighting mass cytometry technology.
VIEW ALLAPPLICATIONS
Webinars
Watch more mass cytometry videos and webinars.
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
Events
Join us for one of our upcoming events or watch on demand.
Mar 28 - Mar 30
Location
Forum Labo in Paris, France
Mar 28 - Mar 30
Location
EACR/OECI (European Association for Cancer Research/Organisation of European Cancer Institutes) Molecular Pathology Approach to Cancer in Bergamo, Italy
Mar 28 - Mar 29
Location
Immuno UK in London, UK
Mar 28
Virtual
Scientist in the Spotlight | Toward identifying immune dysfunction signatures in myelodysplastic neoplasms
Apr 04 - Apr 05
Location
SIG (The Genetics Society) Seed Plant Research Meeting 2023 in Durham, England
Apr 14 - Apr 19
Location
AACR (American Association for Cancer Research) in Orlando, FL
Apr 16 - Apr 18
Location
CIB (CROP Innovation & Business) in Amsterdam, Netherlands
Apr 19 - Apr 21
Location
SIC 2023 | XVII Congress of the Iberian Society of Cytometry in Madrid, Spain
Apr 20 - Apr 22
Location
35th Tumor Genetic Workshop 2023 in Rostock, Germany
Apr 20 - Apr 23
Location
DGfI TIMO XVI (German Society for Immunology) in Halle, Germany
Resources
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Protocols
Tools


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Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.