Cytometry
Overview
The combination of Standard BioTools™ CyTOF® technology and Maxpar® reagents provides you with the world’s most powerful flow cytometric system available today. It has opened new doors to discovery by empowering researchers to interrogate more than 50 markers simultaneously on millions of individual cells to unveil new cell types, functions and biomarkers in immunology, cancer, stem cells and more.
Explore the multiple Maxpar reagent options that make the tedious panel design and validations of fluorescence cytometry a thing of the past.
No CyTOF? No problem!
We can help you convert your fluorescence panel and find a service provider.
Best-in-Class Human Immune Profiling
Eight 40-plus-marker panels enable in-depth profiling of T, B, NK and myeloid cells starting from only 300 μL of whole blood or 3 x 106 of PBMC.
Start with the 30-marker, dry-format Maxpar® Direct™ Immune Profiling Assay™ that enumerates 37 major immune cell populations with proven run-to-run and site-to-site reproducibility. Then layer on any of 9 mixed and matched Maxpar Direct Expansion Panels to formulate ready-to-go experiments profiling cytokines, cytotoxic mediators and other key functional epitopes, all in a single tube. Maxpar Pathsetter™ software provides you with a detailed analytic report including population statistics, 2D data plots and staining quality assessment in minutes.
Maxpar Panel Kits
The 21 Maxpar panel kits for flow cytometry consist of optimized panels of antibodies in liquid format that measure up to 34 surface or intracellular targets on human or mouse cells. Panel kits target specific applications such as cell cycle and signaling pathways, or phenotypic and functional markers associated with specific cell types including stem cells, T and B cells or monocytes/macrophages. Kits include all buffers and other reagents needed to perform 25 tests.
Kits can be combined with each other or individual antibodies to rapidly create customized panels measuring 35 or more parameters simultaneously.
Cell Profiling
Cell profiling with CyTOF allows investigators to identify multiple target types anywhere in the cell, often simultaneously. Available markers include those for:
- Cell-surface targets
- Signaling and transcription factors
- Cytokines
- Cell death and apoptosis
- Cell cycle and proliferation
- Structure and cytoskeleton
- Enzymatic activity
Optimized protocols and detailed technical assistance are available for any combination of targets.
Metal-Tagged Reagents
Cellular targets in liquid suspension can be labeled with more than 50 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands. Each reagent is detected and quantified with cytometry by time-of-flight mass spectrometry in the CyTOF system. The high purity and choice of metal isotopes ensure minimal background noise from signal overlap or endogenous cellular components. Our catalog is stocked with over 800 antibodies detecting more than 400 unique human or mouse targets. If a desired antibody is not yet present in our catalog, you can make it yourself using our metal-labeling kits or custom order biologically validated material using our Custom Conjugation Services.
Sample Barcoding
Sample barcoding is a powerful tool offering increased throughput, improved protocol consistency and enhanced data quality. Barcoding also enables efficient use of small precious samples through multiplexing and may provide cost savings through reduced consumption of reagents. Two approaches are available for CyTOF:
- Cell-ID™ 20-Plex Pd Barcoding Kit for fixed cell samples
- Maxpar anti-human CD45 antibodies for live-cell samples
Custom Panels
Optimize your panel using the web-based Maxpar Panel Designer app, an online tool for building panels with Standard BioTools metal-conjugated antibodies or custom conjugates. Panel Designer calculates signal overlap and utilizes a specialized algorithm to adjust metal-tagged targets to maximize performance. Export and share panels with collaborators or save for future import into our CyTOF systems.
Custom Antibody Conjugation
When your study calls for new antibodies or specific antibody configurations, consult our team of experts for Custom Conjugation Services to tailor your antibody panels for collecting high-impact data. Our Custom Conjugation Services speed you from design to discovery with made-to-order metal labeling for antibodies not currently listed in the Maxpar catalog, or alternative metal tagging for all listed items. Choose from 42 non-overlapping pure metal tags.
Buffers and Solutions
Maxpar buffers provide optimal and consistent staining conditions for external, cytosolic and nuclear targets, while calibration beads enhance data quality and support intersample analysis.
Parts and Accessories
Find air filters, O-rings, torch assemblies, vacuum pump oil and more in our online catalog. It’s your digital source for replacement parts and product refills for all CyTOF systems.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.