Hyperion XTi Imaging System
Introducing the Hyperion XTi™ Imaging System, the fastest and most reliable workflow for high-plex imaging.
Discover the throughput and precision that is uniquely designed for translational researchers.
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We talked about IMC™ and the new Hyperion XTi Imaging System with Hartland Jackson, PhD, and his answers were so brilliant, we just had to share.
Reasons to choose the Hyperion XTi Imaging System
Change the way you look at cells. Accurately visualize and quantify cells to reveal mechanisms of action that impact disease progression and therapeutic outcomes.
Technology
Powered by proven Imaging Mass Cytometry™ (IMC™), Hyperion™ XTi is a next-generation imaging system with 5x the speed and an unmatched limit of detection to accurately quantify and visualize the tissue microenvironment.
IMC is the only technology with
No autofluorescence interference to image any tissue type
40-plus markers imaged simultaneously to get results faster
Protein and RNA co-detection for deeper insights
Integrated cell segmentation for faster interpretation
Batch staining of all slides for high-volume studies
Dual imaging and flow cytometry mode to maximize investment
Learn more about the power of Imaging Mass Cytometry.
Hyperion XTi reliably visualizes lower-expressing markers like PD-L1. Image represents lung squamous cell carcinoma.WORKFLOW
Get results faster
Hyperion™ Imaging Systems use a one-step staining and detection approach that enables samples to be simultaneously stained, acquired and analyzed.
1
Modularized panels
Swap markers without panel revalidation.
2
Simultaneous staining
Stain 40-plus markers for all slides at once.
3
One-step detection
Simultaneous imaging of 40-plus markers, including protein and RNA.
4
Precise signals
Image any tissue without autofluorescence.
5
Real-time analysis
Visualize 40-plus markers in 30 minutes.
Need to ship or store slides?
- All-at-once batch staining of all slides to reduce technical variation
- Acquire at any time from shipped and/or stored slides that have been stained
- Analyze previously banked tissue slides to be correlated to known clinical outcomes
A workflow ideal for high-volume samples,
clinical research trials and multi-site studies
Download this comprehensive brochure to learn more about Hyperion XTi Imaging System.
REAGENTS
Flexible options to meet your research needs
The immune system is complex.
Experiments don’t have to be.
Design your panel in days, not months.
Easily customize targets of interest.
1. Start with ready-to-go human and mouse panels
2. Easily add the Maxpar IMC Cell Segmentation Kit to your panel to solve the most important step in spatial biology.
3. Want to further customize your panel? Easily swap targets of interest from an ever-growing catalog of Maxpar IMC antibodies and panels.
Learn more about tissue imaging reagents
View More PanelsMaxpar® Human Immuno-Oncology IMC Panel Kit
The Maxpar Human Immuno-Oncology IMC Panel Kit (201508) enables interrogation of the tumor microenvironment including activated tumor- infiltrating lymphocytes in FFPE tissue sections using 18-plex IMC. This panel kit includes 17 pathologist- verified antibodies and a nucleic acid stain. The kit combines the Maxpar Human Tumor-Infiltrating Lymphocytes IMC Panel Kit (201506), Maxpar Human Immune Activation IMC Panel Kit (201503), and Maxpar Human Tissue Architecture IMC Panel Kit (201507).
Maxpar® Human Tissue Architecture IMC Panel Kit
The Human Tissue Architecture IMC Panel Kit (201507) enables 6-plex staining of tissue architecture, including stroma, epithelial tissue, and vasculature. This panel kit includes 5 pathologist-verified antibodies and a nucleic acid stain.
Maxpar® Human Tumor-Infiltrating Lymphocytes IMC Panel Kit
The Human Tumor-Infiltrating Lymphocytes IMC Panel Kit (201506) enables 9-plex staining of epithelial cells; phenotyping of T cells (helper, cytotoxic, memory, and regulatory), B cells, and monocytes/macrophages; and identification of potential CD68+ tumor cells. This panel kit includes 8 pathologist-verified antibodies and a nucleic acid stain.
Maxpar® Human Immune Activation IMC Panel Kit
The Maxpar® Human Immune Activation IMC™ Panel Kit enables 5-plex phenotyping of activated T cells in formalin-fixed, paraffin-embedded tissue sections using Imaging Mass Cytometry™. This panel kit includes 4 antibodies and a nucleic acid stain, and leaves 31 open detection channels. This kit can be combined with the Maxpar Human Tissue Architecture and Maxpar Human Tumor-Infiltrating Lymphocytes IMC Panel Kits. This panel kit includes pre-sourced, pre-tagged, and verified antibodies.Target Clone Metal Granzyme BEPR20129-217167ErKi-67B56168ErPD-1EPR4877(2)165HoPD-L1SP142150NdNucleic acid191Ir/193Ir
GET MORE INFORMATION
Interested in Standard BioTools™ Imaging Mass Cytometry products or need a quote? Contact our sales team for product orders, quotes or other inquiries.
Publications
Learn about newly discovered translational insights and find links to prominent examples of researchers’ work to learn why IMC is so valued for top spatial biology applications. Visit the IMC Trending Topics and Publications page to see articles, tips, news and the latest IMC bibliography.
Learn more
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Applications
Hartland Jackson, PhD
Entering an era of new biology
Daniel Schulz, PhD
Discovering a chemokine’s purpose in fighting tumors using RNA and protein co-detection
MCD Viewer
MCD™ Viewer is post-acquisition data processing software that allows users to visualize, review, and export Imaging Mass Cytometry™ data acquired with the Hyperion™ Imaging System and CyTOF® Softwa...
Visiopharm Software
As high-plex IMC™ data continues to stretch our abilities to interrogate the tumor microenvironment, finding meaningful differences in your data becomes an ever more data-heavy and daunting challen...
ilastik
ilastik is an open source software for image analysis and machine learning. Using machine learning, ilastik software trains custom workflows for accurate cell segmentation and classification.
histoCAT
The histoCAT™ software is an innovative computational Imaging Mass Cytometry™ analysis toolbox that enables comprehensive analysis of cellular phenotypes and their interrelationships within the spa...
Specifications
Hyperion XTi is a high-plex imaging system capable of analyzing 40-plus protein markers at subcellular resolution in frozen or formalin-fixed, paraffin-embedded (FFPE) tissue sections. With the ability to utilize up to 135 channels to detect additional parameters, Hyperion XTi is ideal to meet researcher needs today and well into the future.
Instrument Specifications
Channels
135
Mass range
75–209 amu
Abundance sensitivity
<0.3% 141 Pr (M+1 for 140Ce) <0.3% 174 Hf (M–1 for 175 Lu)
Frequency
800 pixels/sec
Detection limit
≥250 copies per μm2
Dynamic range
4.5 orders of magnitude
Calibration
Automated
Operating system
Windows® 10 Enterprise LTSC
Data storage
3.6 TB
Switch time (between modes)
12 hr
Cross-cell contamination (redeposition)
≤2% (selected direction)
Crosstalk pixel to pixel
≤25%
Wet tissue thickness for full ablation
≤7 μm
Maximum addressable sample size
15 mm x 55 mm
Optical view of sample
≥250 µm x 250 µm
File type
TXT, multipage TIFF, OME-TIFF, MCD
Scan rate
≥2 mm2/hr
Data Size*
MCD
4–5 MB/channel
Hyperion XTi Tissue Imager Dimensions**
Width
58 cm (23 in)
Height
132 cm (52 in)
Depth
58 cm (23 in)
Weight
192 kg (423 lb)
* 1,000 x 1,000 µm region of interest (ROI)
** The Hyperion XTi Tissue Imager is connected to the CyTOF XT™ instrument.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.