CyTOF XT
Reimagine cytometry
Fully automated and affordable cytometry by time-of-flight. Simplify and standardize your deep single-cell profiling studies.
No CyTOF? No problem.
We can help you convert your fluorescence panel and find a service provider.
Technology
XTra Autonomy
CyTOF® XT automation expands what your lab can accomplish by simplifying daily workflows, reducing operator time.
XTra Efficiency
Simplified front-end assembly, bottle tray for solutions and automated tuning minimize maintenance and calibration.
XTra Clarity
The precision of measuring marker expression using mass cytometry is unmatched by fluorescence cytometry methods. Cleaner signals translate to more accurate data.
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD, discusses how he is implementing the new high-throughput, high-dimensional CyTOF XT™ cytometer to advance his latest scientific projects.
We acquired the CyTOF XT to expand our capabilities, providing rich data to help us better understand the incredibly complex human immune system, with advancements in throughput, automation and time to results. ”
– Petter Brodin, MD, PhD | Professor of Pediatric Immunology, Imperial College London, Karolinska Institutet
CyTOF XT™: The NeXT Evolution
Preview the new CyTOF XT.
Workflow
1
Design Panel
Easily build panels with 20 to 50 or more metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or begin with our pre-built panels.
2
Load
Up to 13 individual samples can be loaded in the chilled sample carousel for a single batch run.
3
Resuspend
The sample pellet is automatically resuspended and calibration beads added immediately prior to acquisition of each sample.
4
Acquire
Up to 13 different panels and sample types can be acquired unattended with preconfigured acquisition templates on the CyTOF XT.
5
Analyze
FCS 3.1 files can be analyzed with any capable software including FCS Express™ and Cytobank. Maxpar Pathsetter™ provides automated immune profiling data analysis.
Webinars
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
CyTOF Software v8.0
CyTOF® Software v8.0 offers support for cell suspension-based sample analysis to the CyTOF XT system. The software has the built-in capability for automated calibration, batch sample acquisition, a...
CyTOF Software (Non-acquisition workstations only)
CyTOF® Software offers support for imaging analysis to Hyperion™ Imaging System users.
CyTOF Software
CyTOF® Software offers support for the imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sam...
Publications
Powered by Bioz
Support
A critical success factor when adopting high-performance technology is acquiring the proficiency and support necessary to accelerate your research and discovery initiatives. Standard BioTools™ PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources.
Maxpar® Direct™ Immune Profiling Assay™ 30 Marker—25 Tests
Cat. No. 201334 replaces Cat. No. 201325. The Maxpar® Direct™ Immune Profiling Assay™ is a dry 30-marker antibody panel designed and optimized for deep immune profiling of human peripheral whole blood and PBMC. The assay enables comprehensive identification and characterization of 37 immune cell populations.
Cell-ID™ 20-Plex Pd Barcoding Kit
The kit enables unique barcoding of 20 samples so they can be combined and subsequently stained and acquired as one multiplexed sample, followed by software debarcoding and individual sample analysis. Multiplexing samples improves data quality, since the 20 samples are stained, processed, and acquired as one sample, eliminating sample-specific staining and data collection variation.
Maxpar® Cell Acquisition Solution Plus for CyTOF® XT - 1000 mL
Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems.
EQ™ Six Element Calibration Beads—100 mL
The 6-element EQ6 Beads solution is intended for the following uses on the CyTOF XT™ system. Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems.
Specifications
CyTOF XT System
Channels
135
Mass range
75–209 amu
Abundance sensitivity
Instrument response
500 159Tb dual counts/EQ™ Six Element Calibration Beads
Dynamic range
4.5 orders of magnitude
Calibration
Automated
Operating system
Windows® 10 Enterprise LTSC
Sample introduction
Dual syringe drive
Flow rate
30 µL/min
Carryover
Sample format
12 x 5 mL tube positions 1 x 15 mL tube positions
Reagent reservoir
Maxpar® Cell Acquisition Solution Plus for CyTOF® XT 1L Maxpar Water 500 mL Maxpar Washing Solution for CyTOF XT™ 500 mL
Workstation
CPU
Intel® Core™ i7
RAM
GB DDR
Data storage
3.6 TB
Power supply
550W
Monitor
86 cm (34 in) LED
Keyboard and mouse
Wired
Data file specifications
.fcs
4 bytes/channel/event
.lmd
Compressed format. 5–20 GB/hr (typical)
_SPR.fcs
3.82 MB/hr
_Beads.fcs
0.91 MB/hr (typical)
_BG.fcs
0.48 MB/hr
_Processed.fcs
4 bytes/channel/event
CyTOF XT instrument dimensions
Width
93 cm (37 in)
Height
135 cm (53 in)
Depth
75 cm (30 in)
Weight
288 kg (635 lb)
Resources
Videos
Flyers & brochures
Technical documents
Frequently asked questiions
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.