Dual Mode Use of CyTOF Technology
Single instrument for high-multiplex analysis in tissue samples or cells in solution
The Hyperion™ Imaging System is composed of a Hyperion Tissue Imager attached to a Helios™ instrument*. This system has the capability to perform high-multiplex characterization spanning cell suspension and tissue samples using proven CyTOF™ technology. Convenient switching between tissue imaging and suspension modes offers benefits to both core labs that offer mass cytometry and Imaging Mass Cytometry™ services and to translational researchers who need data from multiple sample types or who want to add in-depth spatial data to their work previously performed using disaggregated tissue samples analyzed by mass or flow cytometry.
Translational researchers may want to compare different samples to get more comprehensive datasets that can contribute to greater research insights. For instance, Kevin Kelley, MD, PhD, an Associate Professor of Clinical Medicine at the University of Southern California, commented “the Hyperion Imaging System offers the capability to assess the characteristics of both circulating and tissue cells in a multiplex manner offering a substantial return on investment for the cancer researcher.”
One example of dual mode use is presented in an excellent webcast, Mapping Immunity Across Time, Space and Disease State. Hear Thomas Ashhurst, PhD, High‐Dimensional Cytometry Specialist in the Sydney Cytometry Facility, explain how he applies both mass cytometry and Imaging Mass Cytometry technology for immune cell characterization to evaluate the evolving immune response over time in viral inflammatory responses.
Other information resources on dual mode use:
- An organizational approach to using multiple tools including both mass cytometry and Imaging Mass Cytometry technology to contribute to understanding of cancer and other diseases
- How a research team characterized phenotypic progression in human B cells in suspension using mass cytometry and then used Imaging Mass Cytometry technology to visualize microanatomical distribution of the same cell type
- How dual mode technology enables understanding of disease progression and aids biomarker discovery
Is your core taking advantage of a dual mode system?
If not, ask your sales representative how you can add a Hyperion Tissue Imager to your existing Helios instrument and increase your return on investment. Run suspension samples during the day, and let the automated capabilities of the Hyperion Imaging System help you run IMC samples overnight and over weekends.
Watch this video to learn why Phil Hobson, PhD, Deputy Head of the flow core lab at the Francis Crick Institute, added two Hyperion Imaging Systems in addition to also using a Helios instrument for cell suspension analysis.
customer stories
Learn more about how our customers are leveraging Standard BioTools™ technology.
Yongpan Yan, PhD
An approach that combines molecular biology, immunology and cell biology tools to uniquely
Rebecca Ihrie, PhD
How CyTOF technology enabled a look into the heterogeneity of glioblastoma.
Xianting Ding, PhD
High-dimensional single-cell analysis enhances precision medicine
* The new CyTOF XT system functions only in suspension mode and has important advantages over the Helios instrument for suspension-only users. Talk with your sales representative to determine whether a CyTOF XT system or a Hyperion Imaging System is right for you.
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