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Conjugation Services

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Maxpar Antibody Conjugation Service for Flow Cytometry and Imaging Mass Cytometry

Expand and accelerate your research with isotope-conjugated antibodies made by experts

The use of metal-labeled antibodies as part of CyTOF® technology is the most comprehensive way to profile cell phenotype and function for cells in suspension or in tissue samples, enabling simultaneous detection of over 40 cellular targets for imaging or 50 cellular targets for suspension in every precious sample.

Whether you’re building a mid-range or high-plex CyTOF panel, we offer the extensive Standard BioTools™ catalog of preconjugated antibodies and antibody panel kits to help get you there.

We understand and can help with your panel design needs. If you have a proprietary antibody to add to your panel or require customized design, our Maxpar® Antibody Conjugation Service is committed to your success.

Select the desired isotope from our 51 high-purity metal tags, decide which confirmation method you prefer – biochemical analysis or more extensive biological functional testing – and place your order.

After our expert scientists perform the conjugation and analysis, we’ll send your antibody conjugate along with a detailed analytic report.

 

Save time and resources

Let our experts do the labeling and quality control for you.

 

 

Flexible

Extend your mass cytometry panels by adding metal-labeled targets not yet available in the Maxpar catalog.

Expertise

Conjugation service performed by our professional technicians ensures product with consistently high-quality and reliable performance.

Custom Conjugation Workflow

Custom Conjugation Services Metal Options for Flow Cytometry and Imaging Mass Cytometry

 

Flow Cytometry (51 metal options)

Metal Isotope
Y 89
Cd 106
Cd 110
Cd 111
Cd 112
Cd 113
Cd 114
*In 115
Cd 116
*La 139
Pr 141
Nd 142
Nd 143
Nd 144
Nd 145
Nd 146
Sm 147
Metal Isotope
Nd 148
Sm 149
Nd 150
Eu 151
Sm 152
Eu 153
Sm 154
Gd 155
Gd 156
Gd 158
Tb 159
Gd 160
Dy 161
Dy 162
Dy 163
Dy 164
Ho 165
Metal Isotope
Er 166
Er 167
Er 168
Tm 169
Er 170
Yb 171
Yb 172
Yb 173
Yb 174
Lu 175
Yb 176
Pt 194
Pt 195
Pt 196
*Au 197
Pt 198
Bi 209
Imaging Mass Cytometry  (40 metal options)

Metal Isotope
*Y 89
   
   
   
   
   
   
*In 115
   
   
Pr 141
Nd 142
Nd 143
Nd 144
Nd 145
Nd 146
Sm 147
Metal Isotope
Nd 148
Sm 149
Nd 150
Eu 151
Sm 152
Eu 153
Sm 154
Gd 155
Gd 156
Gd 158
Tb 159
Gd 160
Dy 161
Dy 162
Dy 163
Dy 164
Ho 165
Metal Isotope
Er 166
Er 167
Er 168
Tm 169
Er 170
Yb 171
Yb 172
Yb 173
Yb 174
Lu 175
Yb 176
   
Pt 195
Pt 196
   
Pt 198
   

 

*New metals added to the catalog are indicated by an asterisk and with yellow highlighting.

 

Custom Conjugation Services Specifications and Ordering Information

 

 
With Biochemical Confirmation
With Biological Confirmation*
Biochemical analysis provided
Biological confirmation provided*
Staining profile and titer data
Timeline 3 to 4 weeks from receipt of antibody 5 to 6 weeks from receipt of antibody
Flow Cytometry Antibodies
Scale: standard
Antibody input: 200 µg
Buffer: purified, protein-free buffer
PN 3999999-2
Minimum yield: 40 µg
PN 3999999-1
Yield: 100 tests (1–3 x 106 cells)
Scale: bulk
Antibody input: variable mass
Buffer: purified, protein-free buffer
PN 3999999-7
Minimum yield: 200 µg
PN 3999999-8
Minimum yield: 600 µg
PN 3999999-5
Yield: 100 tests (1–3 x 106 cells)
Extra 100-test vial
Imaging Mass Cytometry™ Antibodies
Scale: standard
Antibody input: 200 µg
Buffer: purified, protein-free buffer
PN 3999999-2D
Minimum yield: 40 µg
 
Scale: bulk
Antibody input: variable mass
Buffer: purified, protein-free buffer
PN 3999999-7D
Minimum yield: 200 µg
PN 3999999-8D
Minimum yield: 600 µg
 

* Not available for antibodies used in Imaging Mass Cytometry

Ligands and buffer suitable for custom conjugation – IgG monoclonal and polyclonal antibodies are routinely conjugated. The feasibility of conjugating other antibody isotypes and proteins is considered on a case-by-case basis. To facilitate labeling, ligands must be provided purified, unconjugated and in a protein-free buffer at minimum concentration of 0.5 mg/mL.

Source of antibodies – Antibodies to be custom conjugated may be either provided by the customer or purchased from a commercial source. Where Standard BioTools purchases an antibody on behalf of a customer, the cost is quoted and billed as a separate line item, PN 3999999-3, Antibody Material Handling Charge.

Quality control – All custom conjugations are subject to rigorous biochemical confirmation, including inductively coupled plasma mass spectrometry, determination of concentration and yield and assessment of ratio of attachment of metal atoms to antibody. Optionally available biological confirmation service involves empirical testing by CyTOF on cells, with optimal titer determined and the conjugate formulated for delivery in a 100-test vial.

Contact us to learn more about Maxpar Custom Antibody Conjugation Services.

 


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