APPLICATION NOTE

Unraveling Metabolic Reprogramming in Cancer with Spatial Proteomics

Modular Imaging Mass Cytometry™ panels identify metabolic states of tumor and immune cells in the tissue microenvironment

 

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Unraveling Metabolic Reprogramming in Cancer with Spatial Proteomics

Read about revolutionary insights into cancer metabolism.

What you'll discover in this application note:

  • Details about a breast adenocarcinoma case study with high-resolution IMC™ imaging
  • A step-by-step methodology for combining immuno-oncology and metabolism panels
  • Quantitative analysis techniques using QuPath for spatial proximity studies
  • Clinical implications for immunotherapy response prediction
  • Guidelines for implementing 43-marker comprehensive tissue analysis

Technical breakthrough: Modular IMC panels

The Human Cell Metabolism IMC Panel targets seven key metabolic markers covering six critical pathways: glucose metabolism, fatty acid oxidation, lactate metabolism, TCA cycle, oxidative phosphorylation and mTOR signaling.

  • Spatially resolved metabolic profiling reveals how tumor cells differentially utilize glucose and fatty acid oxidation to fuel growth
  • Hot vs. cold tumor identification is based on immune infiltration patterns associated with specific metabolic activities
  • Single-cell resolution analysis distinguishes metabolic zones and associates immune cell positioning with tumor metabolic states
  • Therapeutic resistance mechanisms are uncovered through comprehensive spatial and metabolic profiling
  • Precision oncology applications are enabled by detailed metabolic pathway analysis in tissue microenvironments

Immune cells preferentially infiltrate tumor areas containing fatty acid oxidizing tumor cells, revealing critical spatial relationships that could inform immunotherapy strategies.