b'IntroductionUnderstanding the cellular and spatial composition of tissues is crucial for interpreting neural disease origin, progression, prognosis and treatment options. Imaging Mass Cytometry (IMC) is a spatial biology technology that can uncover novel phenotypes and identify therapeutic targets that may be relevant to developing biomarkers and future treatment strategies in neuro-oncology and neurodegenerative studies. Unlike traditional cyclic fluorescent methods, IMC technology can uncover the spatial distribution of 40-plusImaging Mass Cytometry distinct protein markers simultaneously without tissuetechnology enables highly degradation and autofluorescence artifacts usuallyspecific detection of surface observed in brain tissue. This lookbook showcasesand intracellular targets translational and clinical applications of multiplexed tissuewithout interference from analysis using IMC technology. autofluorescent tissues. KEY TAKEAWAYSUsing a combination of imaging modes and ready-to-go high-plex panels onthe same tissue section provides researchers with more flexibility to resolve the distribution of neural and non-neural cell lineages.Rapid imaging modes reveal key biological insights within spatial context that are relevant for developing potential diagnostic and therapeutic applications.NEURO-ONCOLOGYPARKINSONS DISEASE 2 mmALZHEIMERS DISEASEMULTIPLE SCLEROSISPROTEINOPATHIESSYNTAU MIXED PATHOLOGY Ready-to-usePreview Mode Cell Mode Tissue Modepanels Number of markers: 42Number of markers: 42 Number of markers: 42Fast high-parameter panelAcquisition time: 20 minutes Acquisition time: 2 hours Acquisition time:design by combiningSample: normal mouse brainSample: normal mouse brain5 hours and 50 minutes pre-optimized panels with(13 mm x 15 mm) (2 mm x 2 mm) Sample: mouse glioblastoma other relevant IMC panels.Resolution: 1 mbrain (24 mm x 16 mm) Resolution: 5 m'