Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1

Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2

Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3

Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4

Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
PUBLICATIONS TRENDING NOW
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.

March 2022: Maxpar Direct Immune Profiling Assay
January 2022: COVID-19 research update
November 2021: CAR T cells
July 2021: The value of high-parameter cytometry
April 2021: Immuno-oncology
January 2021: Cancer vaccine development
November 2020: Vaccines for infectious disease
July 2020: COVID-19 disease research
Browse the latest research highlighting mass cytometry technology.
VIEW ALLAPPLICATIONS
Webinars
Watch more mass cytometry videos and webinars.
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
Events
Join us for one of our upcoming events or watch on demand.
Feb 23 - Feb 26
Location
Aquaculture America in New Orleans, LA
Mar 05 - Mar 10
Location
DGfI Spring School on Immunology in Ettal, Bavaria
Mar 07 - Mar 09
Location
GPZ/GPW (Working Group Seed Science and Certification) & VDLUFA (Section IV Seeds) Seed Symposium 2023 in Nossen, Germany
Mar 09 - Mar 11
Location
Congress of the Association of Morphologists and the College of Histologists, Embryologists and Cytogeneticists in Lille, France
Mar 09 - Mar 10
Location
7th Annual Markets and Markets Next-Gen Immuno-Oncology Conference in London, UK
Mar 13 - Mar 15
Location
UNIGE (Conference of the International PhD Program in Life Sciences of The University of Geneva) in Geneva, Switzerland
Mar 13 - Mar 15
Location
XXI Scientific Convention in Riva del Garda, Italy
Mar 16 - Mar 17
Location
Brest Mass Days in Brest, France
Mar 20 - Mar 23
Location
10th Gene Quantification Event qPCR, dPCR and NGS in Freising-Weihenstephan, Germany
Mar 28 - Mar 30
Location
Forum Labo in Paris, France
Resources
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Protocols
Tools


No CyTOF? No problem!
We can help you convert your fluorescence panel and find a service provider.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.