Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1
Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2
Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3
Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4
Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
TRENDING NOW PUBLICATIONS TOPICS
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.
November 2023 | NEW
CyTOF for Drug Development
February 2023
CyTOF for CAR Therapy
March 2022
Maxpar Direct Immune Profiling Assay
January 2022
COVID-19 research update
November 2021
CAR T cells
July 2021
The value of high-parameter cytometry
April 2021
Immuno-oncology
January 2021
Cancer vaccine development
November 2020
Vaccines for infectious disease
July 2020
COVID-19 disease research
APPLICATIONS
Webinars
Watch more mass cytometry videos and webinars.
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Katie Vowell, PhD
Principal Investigator – Immunology, GlaxoSmithKline
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
Events
Join us for one of our upcoming events or watch on demand.
VIEW MASS CYTOMETRY EVENTS
VIEW ALL EVENTS
Nov 27 - Dec 02
Location
IUIS (International Union of Immunological Societies) Meeting 2023 in Cape Town, South Africa
Nov 28
Webinar
Scientist in the Spotlight | Fabio Luciani | Analysis of Plasticity of CAR Cells in Patients
Dec 04 - Dec 07
Location
British Society of Immunology in Belfast, UK
Dec 04 - Dec 08
Location
ASI 2023 (Annual Scientific Meeting of the Australian and New Zealand Society for Immunology) in Auckland, New Zealand
Dec 12
Webinar
Scientist in the Spotlight | Chris Tape | Single-cell Signalling Analysis of Tumour Microenvironment Organoids
Jan 12 - Jan 17
Location
International PAG 31 (Plant & Animal Genome) in San Diego, CA
Jan 22 - Jan 24
Location
InFORM (Integrated Foodborne Outbreak Resonse & Management) in Washington DC
Jan 24 - Jan 26
Location
PMWC (Precision Medicine World Conference) 2024 in Silicon Valley, CA
Apr 05 - Apr 10
Location
AACR (American Association for Cancer Research) in San Diego, CA
Resources
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Protocols
Tools
RELATED BLOG POSTS
- Five reasons to go beyond the limits of fluorescence with CyTOF technology
With fluorescence-based cytometry, designing high-parameter experiments can be challenging due to spectral overlap and autofluorescence. Here's how you can avoid those pitfalls and open new doors to discovery.
- What does high-parameter cytometry really mean?
High-parameter cytometry sounds simple enough – indicating more markers are used per assay – but in reality, different high-parameter assays depend on the innate capabilities of a technology.
No CyTOF? No problem!
We can help you convert your fluorescence panel and find a service provider.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.