Helios, a CyTOF System
A powerful high-parameter cytometer and the signal detection platform for the Hyperion Imaging System
The Helios™ mass cytometer enables the sensitive and precise detection of metal-tagged antibodies bound to tissue regions of interest interrogated by the Hyperion™ Tissue Imager.
Helios also serves as a high-dimensional single-cell suspension analysis tool when decoupled from the Hyperion Tissue Imager. Learn more about the unique capabilities of the Hyperion+™ Imaging System to function in dual mode to perform high-multiplex characterization spanning cell suspension and tissue samples using proven CyTOF® technology.
For latest-generation, stand-alone cell suspension mass cytometry, the fully automated CyTOF XT™ provides significant advantages over the Helios system. Reimagine cytometry with CyTOF XT.
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or begin by using pre-built panels.
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. View our support page for staining protocols.
The Helios system provides the same excellent standard of data quality as the latest-generation mass cytometer, CyTOF XT, but requires manual resuspension and loading of samples.
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including but not limited to FCS Express™ and Maxpar Pathsetter™.
CyTOF systems are the only high-parameter cytometers backed by more than 1,500 publications. Covering a wide spectrum of diseases and research applications, this collection of studies demonstrates the versatility and proven performance of mass cytometry for translational and clinical research around the world.
Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering (SPADE and viSNE), heat maps, do...
CyTOF® Software offers support for the imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sam...
FCS Express™ is a fully integrated analysis, statistics, graphing and reporting tool for turning cytometry data into presentation-ready and publication-ready results. Full support for Fluidigm cyto...
Maxpar® Pathsetter™ is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Pr...
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Flyers & brochures
Helios, a CyTOF system
0.3% for 159Tb
600,000 counts/pg 159Tb
4.5 orders of magnitude
Windows® 10 Enterprise
Pneumatic single-tube loader with agitation, up to 5 mL volume
Replicate sample CV (normalized)
Windows 10 Enterprise
OS: 240 GB SSD, Storage: 2 x 8 TB
Intel Core i7-6700 @ 3.4 GHz
32 GB DDR4
Height 34.5 cm (13.6), Width 13.4 cm (5.25), Depth 35.6 cm (14 in), Weight 8 kg (18 lb)
86.3 cm (34 in)
Data file specifications
103 cm (41 in)
132 cm (52 in)
87 cm (35 in)
320 kg (705 lb)
38 cm (15 in)
64 cm (25 in)
67 cm (27 in)
81 kg (178 lb)
Mass Cytometry Technology Learn more about mass cytometry and how it is being used to advance basic and clinical research in oncology, immuno-therapy and infectious disease.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.