The CyTOF XT system
Reimagine cytometry
Fully automated and affordable cytometry by time-of-flight. Simplify and standardize your deep single-cell profiling studies.
CyTOF™ XT: The NeXT Evolution
Preview the new CyTOF XT system
APPLICATIONS
Technology
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF technology to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
- XTra autonomy: CyTOF XT automation expands what your lab can accomplish by simplifying daily workflows and freeing operator time for other tasks
- XTra efficiency: Simplified front-end assembly, a bottle tray for solutions and automated tuning minimize maintenance and calibration
- XTra clarity: The precision of measuring marker expression using mass cytometry is unmatched by fluorescence cytometry methods. Cleaner signals mean data is more accurate.
Jennifer Snyder-Cappione, PhD
Assistant Professor of Virology,
Immunology & Microbiology
Boston University School of Medicine
Overcome the challenges of functional profiling in spectral flow cytometry – with the CyTOF XT system
A must-see session highlighting what could be missing in spectral flow cytometry results. Learn more about a head-to-head comparison of intracellular staining panels using a spectral flow cytometer and the CyTOF XT mass cytometer.
Workflow
1
Design panel
Easily build panels with 20 to 50 or more metal-tagged antibodies and reagents using Maxpar Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or begin with our pre-built panels.
2
Load
Up to 13 individual samples can be loaded in the chilled sample carousel for a single batch run.
3
Resuspend
The sample pellet is automatically resuspended and calibration beads added immediately prior to acquisition of each sample.
4
Acquire
Up to 13 different panels and sample types can be acquired unattended with preconfigured acquisition templates on the CyTOF XT system.
5
Analyze
FCS 3.1 files can be analyzed with any capable software including FCS Express and Cytobank software. Maxpar Pathsetter software provides automated immune profiling data analysis.
GET MORE INFORMATION
Interested in Standard BioTools™ mass cytometry products or need a quote? Contact our sales team for product orders, quotes or other inquiries.
Maxpar Direct Immune Profiling Assay 30 Marker—25 Tests
PN 201334 replaces PN 201325. The Maxpar® Direct™ Immune Profiling Assay™ is a dry 30-marker antibody panel designed and optimized for deep immune profiling of human peripheral whole blood and PBMC. The assay enables comprehensive identification and characterization of 37 immune cell populations.
Cell-ID™ 20-Plex Pd Barcoding Kit
The kit enables unique barcoding of 20 samples so they can be combined and subsequently stained and acquired as one multiplexed sample, followed by software debarcoding and individual sample analysis. Multiplexing samples improves data quality, since the 20 samples are stained, processed, and acquired as one sample, eliminating sample-specific staining and data collection variation.
Maxpar® Cell Acquisition Solution Plus for CyTOF® XT - 1000 mL
Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems.
EQ™ Six Element Calibration Beads—100 mL
The 6-element EQ6 Beads solution is intended for the following uses on the CyTOF XT™ system. Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems.
Resources
Videos
Flyers & brochures
Technical documents
Frequently asked questiions
CyTOF Software v9.2.1
CyTOF™ Software v9.2.1 offers instrument control and data acquisition functionality for both imaging and flow applications with the Hyperion™ XTi Imaging System, CyTOF XT System and CyTOF XT PRO Sy...
Maxpar Pathsetter
Maxpar™ Pathsetter is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Di...
FCS Express
FCS Express is a fully integrated analysis, statistics, graphing and reporting tool for turning cytometry data into presentation-ready and publication-ready results. Full support for Standard...
Specifications
CyTOF XT System
Channels
135
Mass range
75–209 amu
Abundance sensitivity
<0.3% for M+1 with M = 159Tb <0.3% for M–1 with M = 175Lu
Instrument response
500 159Tb dual counts/EQ™ Six Element Calibration Beads
Dynamic range
4.5 orders of magnitude
Calibration
Automated
Operating system
Windows® 10 Enterprise LTSC
Sample introduction
Dual syringe drive
Flow rate
30 µL/min
Carryover
<1%
Sample format
12 x 5 mL tube positions 1 x 15 mL tube positions
Reagent reservoir
Maxpar® Cell Acquisition Solution Plus for CyTOF® XT 1L Maxpar Water 500 mL Maxpar Washing Solution for CyTOF XT™ 500 mL
Workstation
CPU
Intel® Core™ i7
RAM
GB DDR
Data storage
3.6 TB
Power supply
550W
Monitor
86 cm (34 in) LED
Keyboard and mouse
Wired
Data file specifications
.fcs
4 bytes/channel/event
.lmd
Compressed format. 5–20 GB/hr (typical)
_SPR.fcs
3.82 MB/hr
_Beads.fcs
0.91 MB/hr (typical)
_BG.fcs
0.48 MB/hr
_Processed.fcs
4 bytes/channel/event
CyTOF XT instrument dimensions
Width
93 cm (37 in)
Height
135 cm (53 in)
Depth
75 cm (30 in)
Weight
288 kg (635 lb)
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