Area of Interest Immune Profiling  Human Maxpar Direct Immune Profiling System

Immune Profiling 

Maxpar Direct Immune Profiling Assay and Maxpar Pathsetter software

Introducing the first complete sample-to-answer solution for single-cell high-dimensional immune profiling of human PBMC and whole blood, the Maxpar® Direct™ Immune Profiling Assay™ and Maxpar Pathsetter™ software. Each provides the data quality and reproducibility required to capture the highly individual nature of immune response in health and disease.

No CyTOF? No problem.
We can help you convert your fluorescence panel and find a service provider.

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Blue icon illustrating immune profiling application

The technical details: populations, clones and workflows 

Designed as a simple single-tube workflow, the system brings together CyTOF® technology on the CyTOF XT™ and Helios™ mass cytometry systems, a dry-format 30-marker antibody panel and automated Maxpar Pathsetter software, enabling identification of 37 immune cell populations.

Get more details from the Maxpar Direct Immune Profiling flyer

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Single-tube antibody panel design. All antibody clones used in the kit are familiar and industry-proven.

The 37 immune cell subsets identified using the Maxpar Direct Immune Profiling Assay and Maxpar Pathsetter software.

Samples stained with the Maxpar Direct Immune Profiling Assay can be fixed, frozen and stored for analysis at a later time or at another location.

Featured Application

CAR Therapy

With over 2,000 publications and 200 clinical trials, CyTOF® flow cytometry and tissue imaging can be used for a variety of immune and non-immune related diseases. Here we narrow in on an emerging area for high-parameter cytometry.

Chimeric antigen receptor (CAR) cell therapy is a promising breakthrough to significantly improve the management and treatment of cancer. Yet there is much to understand about resistance, toxicity, limited CAR T cell expansion and inadequate persistence in order to gain a stronger foothold in the clinic.

Going beyond the limitations of fluorescence, CyTOF flow cytometry enables simultaneous characterization of CAR cell phenotyping and immune response in a single assay for highly reliable and reproducible results essential for longitudinal, multicenter and clinical trial studies. 

Read the latest Trending Now reviewing recent publications using high-throughput proteomic monitoring of single-cell phenotypes in CAR therapy development

See a selection of publications featuring leading-edge CAR therapy research using Standard BioTools™ technology


The Maxpar Direct Assay: An expandable 30-marker dry-format cytometric assay for deep immune profiling.

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See the benefits of CyTOF XT for immune profiling.

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The Maxpar Direct Immune Profiling Assay was designed with customization in mind.

Choose from 8 ready-to-go 40-plus-marker panel options. Profile T, B, NK and myeloid cells for activation status and cytokine production, all in a single tube.

Download flyer to learn more

Building a 47-marker flow cytometry panel to explore antigen-specific immune responses.

Download application note


Reimagine Flow Cytometry



Gloria Martrus, PhD
Field Application Scientist,
Standard BioTools


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Customization of the Maxpar Direct Immune Profiling Assay and Maxpar Pathsetter for Use in a Biotech Setting

Pedro Estrada

Pedro Estrada, MS
2seventy bio™
Cambridge, MA

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Molecular Characterization of CAR T Cells Using Mass Cytometry

Ilaria Michelozzi

Ilaria Michelozzi
Postdoctoral Researcher,
University College London

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Bringing Immune Profiling One Step Closer to Clinics with Cytometry by Time-of-Flight (CyTOF)

Sasidhar Vemula, PhD

Sasidhar Vemula, PhD
Lead Scientist
Translational Biomarker Solutions
Labcorp Drug Development
Vanderbilt University

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CyTOF in the Cancer Immune Monitoring and Analysis Centers (CIMAC): Standardization and Harmonization

Holden Maecker

Holden Maecker, PhD
Professor of Microbiology and Immunology
Director of the Human Immune Monitoring Center Stanford University

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A Complete Immune Monitoring Solution with CyTOF: Ideal for Pandemics and Beyond

Frederik De Smet

Frederik De Smet, MSc, PhD
Assistant Professor
University of Leuven, Belgium

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Customer Stories

Learn more about how our customers are leveraging the Maxpar Direct Immune Profiling Assay.

Lori Turner, PhD


A quick pivot to COVID-19 research with CyTOF

Benjamin Terrier, MD, PhD


Discovering a hallmark phenotype of COVID-19


Don’t have a CyTOF system? We can help!

Maxpar Direct Assays are still the best option for your study if you want convenience, flexibility and consistency.

Maxpar reagents are so robust you can stain, freeze, store and ship your samples for analysis to a CyTOF service provider.



Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.