Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1
Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2
Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3
Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4
Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
TRENDING NOW PUBLICATIONS TOPICS
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.
Prevention and treatment of neurological disease
Vaccine development and efficacy testing
CyTOF for Drug Development
CyTOF for CAR Therapy
Maxpar Direct Immune Profiling Assay
COVID-19 research update
CAR T cells
The value of high-parameter cytometry
Immuno-oncology
Cancer vaccine development
Vaccines for infectious disease
COVID-19 disease research
APPLICATIONS
WEBINARS
Jennifer Snyder-Cappione, PhD
Assistant Professor of Virology,
Immunology & Microbiology
Boston University School of Medicine
Overcome the challenges of functional profiling in spectral flow cytometry – with CyTOF XT.
A must-see session highlighting what could be missing in spectral flow cytometry results. A head-to-head comparison of intracellular staining panels using a spectral flow cytometer and the CyTOF XT™ mass cytometer.
Watch more mass cytometry videos and webinars.
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Katie Vowell, PhD
Principal Investigator – Immunology, GlaxoSmithKline
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
Events
Join us for one of our upcoming events or watch on demand.
VIEW MASS CYTOMETRY EVENTS
VIEW ALL EVENTS
Jul 25 - Jul 26
Location
JCS 2024 (Japanese Cytokine Society) in Sopporo, Japan
Jul 28 - Aug 01
Location
AAIC (Alzheimer's Association International Conference) in Philadelphia, PA
Aug 18 - Aug 23
Location
EUCARPIA (European Association for Research on Plant Breeding) 22nd General Congress in Leipzig, Germany
Aug 26 - Aug 30
Location
AQUA 2024 (World Aquaculture Society and European Aquaculture Society Joint Meeting) in Copenhagen, Denmark
Sep 01 - Sep 03
Location
EMBO Workshop "The Spatial Biology of Cancer" at The Francis Crick Institute in London, UK
Sep 01 - Sep 04
Location
ECI (7th European Congress of Immunology) in Dublin, Ireland
Sep 09 - Sep 12
Location
2nd Annual Conference on Mass Spectometry Imaging and Integrated Topics in cooperation with DGMS (German Society for Mass Spectrometry) in Münster, Germany
Sep 10 - Sep 13
Location
DGFZ (Germany Society of Cytometry) Annual Meeting in Berlin, Germany
Sep 11
Location
Digital Pathology & AI Congress: Asia in Singapore
Sep 11 - Sep 12
Location
CGDI Shanghai Summit 2024 (Cell & Gene Therapy Development & Innovation) in Shanghai, China
Resources
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Tools
No CyTOF? No problem!
We can help you convert your fluorescence panel and find a service provider.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.